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KO RBC had lower adenylate pools (calculated as ATP+0.5 ADP/[ATP+ADP+AMP]) and altered reduced/oxidized glutathione ratios, consistent with oxidative stress seen in high-caffeine RBC

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Oral administration faces hepatic first-pass metabolism, reducing bioavailability to approximately 4050% of subcutaneous routes, but offers convenience for longer-term studies where daily injections create compliance challenges

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doi:10.1038/s41598-018-29313-w 96

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