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All samples were labeled with N -(2-aminoethyl) rhodamine 6G-amide bis(trifluoroacetate) (R6G) by using the EDC/NHS coupling reaction with a weight ratio of polypeptide to R6G at 20:1 (Fig
2.5 Sample preparation After evaluation of seizure behavior, the blood samples were collected from the hearts of all rats under deep anesthesia using 80 mg/kg ketamine with 10 mg/kg xylazine (Kitazawa et al., 2005
Invest 124 , 44734488 (2014)
International Journal of Obesity and Related Metabolic Disorders
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