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Next, cells were incubated with 0.01 mg/mL primary antibody and then with secondary VectaFluor anti-rabbit IgG Dylight 488 antibody for 30 min at RT

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The two synthetic GPR40-specific compounds AM-1638 and AM-5262, have been found to act as double Gq and Gs agonists but also as positive allosteric modulators, capable to enhance the GLP-1-secreting capabilities of Gq-only agonists such as dietary docosahexaenoic (DHA) and -linolenic acid (ALA), independently of the orthosteric site (265)

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