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Interest broadened sharply after Yang and colleagues (Cell, 2014) used targeted metabolomic profiling across twelve ferroptosis-inducing small molecules and found that glutathione depletion converges on inactivation of the selenoenzyme glutathione peroxidase 4, placing glutathione availability directly upstream of iron-dependent lipid peroxidation

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Western blot Proteins were extracted from Huh7 or Hep3B cells and separated using 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, cat

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