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We found that 100300-kDa filtrate substantially increased GLP-1 secretion and when concentrated filtrates were passed through anion-exchange chromatograph and tested ability of each fraction to induce GLP-1 secretion, specific fractions found to induce GLP-1 secretion in L cells

Acetonitrile and methanol HPLC-grade (Sigma-Aldrich Chemie GmbH, Switzerland), ortho-phosphoric acid (BDH Laboratory Suppliers, England) and double distilled water were used in our study