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Researchers should define the main question before selecting a model: whether the study is testing cytoprotection, migration, proliferation-associated endpoints, angiogenesis, extracellular matrix remodeling, endothelial signaling, or tissue-level recovery

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The plate was then incubated for 5 min at room temperature before measuring the absorption at 590 nm using the Fluostar Optima (BMG LabTech, Ortenberg, Germany)