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Growth hormone levels peak, and the metabolic focus shifts from "doing" to "fixing." Some athletes prefer taking collagen at night because the amino acids like glycine can have a calming effect on the central nervous system, potentially supporting better sleep quality while providing the building blocks for overnight joint and muscle repair

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Cell 151(1):96110

Protein isolation and western blotting For standard western blotting, tissues were lysed in RIPA buffer and extracted protein content was determined using the BCA protein assay (Sigma, St