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Moreover, the pattern of signal bias first demonstrated in HEK293 cells was recapitulated when we expressed SNAP-GLP-1R in INS-1 832/3 cells in which the endogenous GLP-1R was deleted by CRISPR/Cas9 (Naylor et al., 2016), with lixisenatide showing a relative preference for internalisation compared to cAMP signalling (Figure 4C, Supplementary Figure 3A)

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This epigenetic intervention engages in extensive cross-talk, potentially through global demethylation and modulation of microRNA expression, thereby reversing aberrant gene transcription profiles in cancer (65)