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Identity of synthetic AQ-QI and DEAQ-QI was verified by mass spectrometry and the purities were above 95% (Supplementary Figure S1), as determined by HPLC-UV and LC-TOF-MS ( Cell Culture HepG2 cells were cultured in collagen-coated plates and maintained in DMEM containing 10% FBS, 1% penicillin/streptomycin (PAA Laboratories, Austria), 1% ultraglutamine (Lonza, Switzerland) and 1% non-essential amino acids (Sigma-Aldrich, Germany)
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In the study by Fontcuberta-PiSunyer et al., human fibroblasts were directly reprogrammed using adenoviral vectors delivering NEUROG3, PDX1, and MAFA, followed sequentially by PAX4 and NKX2-2
Therefore, the fact remains that the benefits of L-carnitine (as well as those of other supplements), can be indirect and take longer to be extremely visible, for example weeks, unlike supplements that can directly affect sports performance (such as creatine and caffeine), but certainly not less for the systemic effects on the well-being and health of the person who uses it
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