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Isolated cardiomyocytes can reveal cell-autonomous metabolic mechanisms but omit endothelial, fibroblast, immune, neural, and systemic substrate supply

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The partial specific volumes and molecular masses of the proteins were calculated on the basis of their amino acid compositions in SEDFIT ( The same sedimentation velocity (sv) profiles (fluorescence intensity) of AF488-ASPL (final concentration of 0.2 M) in a mixture with VCP (final concentration of 16.8 M) in 50 mM Hepes, 200 mM NaCl, 2 mM ATP, 2 mM MgCl 2 , and 0.5 mM sodium vanadate buffer were also fitted to a two-dimensional size-and-shape distribution, c ( s , f / f 0 ) (with one dimension representing the s distribution and the other the f / f 0 distribution) model