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The zebrafish were individually treated for 40 min in a beaker containing the respective concentrations of NAC, ALC, or DZP (400 mL solution), following the protocol described by Mussulini et al

Next, to examine functional responses to glucose after RETA or RETA-w/d, oral glucose tolerance tests (OGTT) were performed

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Furthermore, its weight loss effect, achieved by reducing fat accumulation, preserves an appropriate amount of muscle mass during weight reduction, preventing muscle atrophy due to nutritional deficiencies and maintaining normal physiological functions and bodily resistance