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To determine the accurate concentrations of [ 15 N 13 ]-CNCbl used for each extraction, a calibrator solution comprising unlabeled CNCbl (50-100 ng/mL) and labeled [ 15 N 13 ]-CNCbl (50-100 ng/mL) was routinely prepared and measured by LC-MS/MS
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Perform a spot test on the inner arm before full application to check for sensitivity
This natural response helps signal fullness and supports blood sugar regulation
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