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Signs of bacterial contamination: Cloudiness (should be clear) Particles or floating material Unusual odor Color change Physical degradation signs Visual changes indicating degradation: Clear solution becomes cloudy Color change (especially copper peptides) Precipitation or crystals forming Separation of layers When in doubt: Discard

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Bile formation and secretion

Runwal P, Pyun J, Newman SA, Mawal C, Bush AI, Koehn LM et al (2025) Ferric ammonium citrate reduces claudin-5 abundance and function in primary mouse brain endothelial cells