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The next day, following three washes in TBST, the membranes were incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (rabbit, A9169, 1:10,000, Sigma) or anti-mouse IgG (mouse, A9044, 1:10,000, Sigma) at room temperature for 1 h

Regulation of SIRT1 and its roles in inflammation

Both peptides appear well tolerated in the limited settings studied, but long-term systemic human safety is uncharacterized for each, and research-grade material carries unverified identity and purity

While LPS exposure alone resulted in a 59% increase in oxidized DCF (a measure of ROS) compared to control cells, pretreatment with GHK-Cu at concentrations of 1, 5, and 10 M significantly decreased ROS production