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GHK-Cu is a copper-complexed tripeptide with the amino acid sequence glycyl-histidyl-lysine (GHK)

Then, samples were diluted with a solution containing phosphate buffer (0.01 mol/L monobasic potassium phosphate/dibasic potassium phosphate, pH 6.85) containing sodium dithionite, and after 10 min at room temperature, absorbance was measured at 420 and 432 nm against a matched curve containing only buffer [21]