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Unlike many animals, humans cannot produce their own Vitamin C

Glutathione not only functions as the substrate for protein S-glutathionylation but can also reverse the process by reducing the cysteine in proteins to which glutathione is disulfide bonded forming oxidized glutathione, GSSG

With the aid of X-ray crystallography and site-directed mutagenesis techniques, the roles of GST active site amino acid residues were identified and evaluated [62, 81]