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Supernatant was removed, and nuclei were resuspended in 1 mL of Nuclei wash buffer, passed through a 40 m FlowMi cell strainer and counted using a Cell Countess II

After ethanol evaporation, 75 L of lysis buffer [0.1% (w/v) BSA, 0.3% (v/v) Tween 20, and 5 mM HEPES, pH 7.4] was added, and 10 L of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences)

Someone may have to be on treatment for over the course of their lifetime, she said