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In PAM212 cells, 12-hour closure was lower but still notable, achieving 87% (GHK-AgNPs) and 90% (GHK-Cu-AgNPs) at 24 hours, both significantly higher than controls

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One possible unifying model is that direct membrane transfer predominates in short-range spread, whereas redox trigger waves and extracellular relay mechanisms govern propagation over medium to long distances, thereby establishing a hierarchy of spatial coupling mechanisms