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Both these methylation reactions are vital for growth and cell reproduction.[9][10] Methionine, a sulfur-containing, essential amino acid, is a precursor of S-adenosylmethionine, a cofactor for one-carbon metabolism and the final methyl donor for the methylation of DNA, RNA, proteins, and phospholipids.[11] The methionine synthase plays a paramount role in the synthesis of nitrogenous bases (purines and pyrimidines) involved in the formation of DNA

Typically, patients with a deficiency might start with more frequent injections, often weekly, and then switch to monthly maintenance doses once levels stabilize

At sacrifice, blood and brain tissues were collected for metabolic, body composition, and transcriptomic analyses