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The crude material was purified by automated flash column chromatography on silica via gradient elution, from 100% dichloromethane to 1% methanol in dichloromethane to produce SS(NHS-CM) 2 (979 mg, 79%) as an off-white foamy solid
doi:10.1016/j.bbr.2019.112359 84
These show increases earlier in the course of deficiency, before hematologic manifestations set in
Serum samples were diluted three times with normal saline before analysis
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