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doi: 10.1677/joe.0.172r007 30 JefferyP

Thus, the ATP depletion and mitochondrial ROS burst observed upon SLC25A39 knockdown are expected to blunt PI3K/AKT signaling and subsequently inhibit mTORC1, driving the observed cell death

3.7 Caspase-3 expression via immunohistochemistry The control and spirulina-treated rats (400) had comparable low expression of caspase-3 in their liver tissue, indicated by the lack of positive brown staining (Figures 5A,D)