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Homogenates were centrifuged at 10,000 g for 20 min at 4 C, and supernatants were collected and subjected to hydrophobic chromatography (C18 SepPak cartridges, Waters Corporation) according to the manufacturers instructions
The OCTN subclade, comprised of OCTN1 (SLC22A4) and OCTN2, are a distinct subgroup of OCTs that primarily catalyse the uptake of the zwitterionic metabolites ergothioneine 13 and carnitine respectively, but are also capable of transporting cations such as tetraethylammonium (TEA) 14,15 indicating a potential dual transport function 15,16
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