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It typically appears in a persons 20s, 30s or 40s
After separation, proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore Corporation, USA, #IPVH00010) and blocked with 5% skim milk powder (Biosharp, Beijing, China, #BS102-500g) for 2 h at room temperature
, may have lipotropic effects
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