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A round black tub was filled with water and divided into four equal regions labeled as north (N), south (S), east (E), and west (W)
While this strategy is more advanced than performing the pull-down assay without crosslinking, freezing protein interactions by crosslinking may make or break the success of a pull-down assay
7:e48924
The authors further use the invasive isolates MC58 and DE13664, and the carrier isolates 711 and 275, revealing a dramatic change in the capability of crossing the Calu3 monolayer and in the number of intracellular bacteria between invasive and carrier isolates (Peters et al., 2024)
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