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Sites Abdomen (2 from navel) Thighs/arms Rotate to avoid irritation Needles 29-31G insulin syringes 0.5-1 mL, length Reconstitution (10 mg Vial) Add 2 mL bacteriostatic water (0.9% benzyl alcohol)

Moreover, persistent hyperglycaemia triggers activation of the (NF-B) pathway and release of pro-inflammatory factors, leading to an imbalance between the pro- and anti-inflammatory networks, resulting in an increase in reactive oxygen species and the production of a large number of inflammatory mediators, which affects the functioning of the mitochondria and leads to neuronal damage and degeneration (29)

Microbial contamination (especially in solution) which not only depletes potency but raises safety risks

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