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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

In standard DXA methodology, lean mass primarily measures non-fat soft tissue - predominantly muscle - rather than bone mineral content, which is reported separately

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Praluent and Repatha are used alongside an exercise and diet plan, as well as other medications, to reduce the risk of these complications