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To analyse proliferation of HCT116 cells, Lipofectamine 2000 (Invitrogen) was used to transfect the shRNA-resistant expression vector of FLAG-PGRMC1 or an empty vector into HCT116 control or PGRMC1-knockdown cells

PMID 2716644

Nancy Shenoi, M.D., assistant professor of psychiatry at Baylor College of Medicine, pointed out gaps in obesity treatment in low- and middle-income countries and rural areas