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Tirzepatide exhibited an affinity for GIPR equivalent to GIP binding, while its affinity for GLP-1R was approximately five times weaker compared to GLP-1. Frontiers in Endocrinology, 2024 This receptor selectivity profile means that impure preparations could disproportionately affect GLP-1R activation measurements, since the compounds GLP-1R activity operates at much higher concentrations (EC 934 pM vs