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Samples were then amplified and sequenced on a NextSeq 500 system (Illumina), where an average of 30 million reads were generated from each sample

The reaction is catalyzed by a family of enzymes called Glutathione S-transferases (GSTs), which facilitate a nucleophilic attack, covalently binding the sulfur atom of glutathione directly to the toxic Phase I metabolite

Furthermore, GSH deals directly with heavy metals and persistent organic pollutants (POPs), direct causes of oxidative stress