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Loren Pickart isolated it from human plasma albumin at the Cardiovascular Research Institute, observing aged liver tissue synthesizing proteins like younger tissue upon exposure

Key observations include: in aged rodents undergoing surgery, there is evidence of mitochondrial swelling and cristae disruption in hippocampal neurons (64)

HP 13 C MR via dissolution dynamic nuclear polarization (dDNP) is an emerging imaging technology that allows direct visualization of metabolic conversion of 13 C-enriched molecules, and has been applied extensively to study metabolism in cells, tissues, animal models, and patients 22,23,24