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To separate MCF derivatives from the reaction mixture, 400 L chloroform was added and vigorously mixed for 10 s followed by the addition of 400 L sodium bicarbonate (50 mM), and mixing for an additional 10 s

While a high-fiber diet was able to increase the density of L cells and proglucagon mRNA levels in the colon of obese and leptin-deficient mice (Jrgensen et al., 2012