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CntA/B is a notoriously hard enzyme to study, but once it was stabilized, we were able to gain insight into how CntA perceives its L-carnitine substrate with a 3D crystal structure model, and by studying the complete electron transfer pathway, we could see how the protein is able to turn over TMA

Targeting activation pathways and inhibitory receptors is a promising therapeutic frontier

In vivo, zinc deficiency does not independently induce ER stress, yet under pharmacologically induced ER stress conditions, zinc-deficient mice exhibit heightened activation of the pro-apoptotic p-eIF2ATF4CHOP axis, accompanied by apoptosis, steatosis and liver injury

Liver samples were lysed rapidly in 20% ice-cold trichloroacetic acid (TCA), and the resulting lysate from each sample was split into 2 identical half-samples containing approximately 200 g proteins each