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Conclusion Vitamin B12 is an essential nutrient important for various physiological processes, including red blood cell formation, DNA synthesis, and nerve function

Research has also linked choline deficiencies to nonalcoholic fatty liver disease [8]

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Altogether, our integrated datasets can provide a robust multispecies IF expression and characterisation framework, quantitatively comparing yield, folding, ligand binding, and antibody crossreactivity, and for now identify bovine IF as a promising nonhuman candidate for oral B12 delivery with minimal interference from antihuman GIF antibodies under our experiment conditions