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Immunolocalization of glutathione S-transferase in the rat uterus

For the cell apoptosis experiment, the ARP1-SRD5A1-KD and H929-SRD5A1-KD cells post 2 g/mL treatment for 48 h at a density of 2 10 5 cells were collected and stained using Annexin-V-Allophycocyanin (Annexin-V-APC) (#640941, BioLegend, San Diego, USA) and PI for 15 min in dark conditions, and the apoptosis of the cells was detected using flow cytometer

We describe these as areas of potential support, not promises, and we set expectations clearly before anyone begins