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In the pathological changes of neonatal HI encephalopathy (HIE), the time of injury and the time of treatment play an important role because of the cascading reaction process and cell changes

To construct the blank vectors for gene overexpression capable of affinity cell sorting, we inserted the coding sequence of GST-EGFP-GPI DAF or GST-EGFP-GPI CEAM7 marker into pcDNA3.1 in place of the neomycin resistance gene and obtained pcDNA3.1-GST DAF and pcDNA3.1-GST CEAM7 (Figure 6A)

Acknowledgements This study is extracted from the master thesis of Motahareh Taghizadeh and was supported by Grant Number of 25345 from the Vice-chancellor for Research Affairs of Shiraz University of Medical Sciences, Shiraz, Iran