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Metabolic/Bariatric Surgery (Third-Line) Indicated for BMI 40 or BMI 35 with obesity-related comorbidities (DM2, HTN, OSA, OA)

Immunohistochemical detection of alpha and beta cells was performed using rabbit anti-insulin (3014, 1:800, Cell Signaling Technology) and mouse anti glucagon (G2654, 1:1,000, Merck) as primary antibodies and goat anti-rabbit AF750 (A21039, 1:100, Invitrogen) and donkey anti-mouse AF555 (A32773, 1:200, Invitrogen) as secondary antibodies

Understanding proper GLP-1 injection technique reduces this risk significantly

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