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Ubiquitination of NS1 confers differential adaptation of Zika virus in mammalian hosts and mosquito vectors
Fecal genomic DNA was extracted using the QIAmp Power Fecal DNA Kit (QIAGEN, Netherlands) according to the manufacturers instructions and quantified using the NanoDrop ND-1000 spectrophotometer (Thermo Fischer Scientific)
In summary, TMAO demonstrates a biphasic protective-damaging mechanism in HF, with its net effect determined by dose thresholds, exposure duration, and pathological context
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