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The in-frame EGFP module at the C-terminal of the GST tag allows the positive cells to be observed under fluorescent microscopy and quantified with flow cytometry
For instance, Bifidobacterium promotes Th1/Th2 cell balance and upregulates the secretion of IFN-, IL-4, and IL-12 from the spleen (161)
Inconsistent results between vials usually trace back to reconstitution variables: slightly different water volumes, different storage conditions, or different ages of the reconstituted solution
