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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

The number of measurable particles per milliliter for each spray experiment performed on each test combination ( measured by MFI )

Palasz E, Wysocka A, Gasiorowska A, Chalimoniuk M, Niewiadomski W, et al

In addition to its ability to float, the house is highly insulated and incorporates a Mechanical Ventilation and Heat Recovery System to keep heating requirements down to a minimum