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A comprehensive panel of antibodies was utilized for Western blotting, targeting phosphorylated and total forms of ERK1/2, p38 MAPK, JNK1/2, and NF-B p65 (Ser536), with -actin serving as a loading control

Recorded as blue lyophilized powder, consistent with the copper content of GHK-Cu

Analysis of adipogenic and osteogenic differentiation of human mesenchymal stem cells Human mesenchymal stem cells (MSC) (Promocell, Heidelberg, Germany) were maintained in culture under basal medium (Promocell) and then induced for adipogenesis and osteogenesis with corresponding differentiation media (Promocell) for 14 days with medium change every 23 days