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One gentle preservative
Im a physician and clinical epidemiologist, and my team and I design and interpret these same kinds of studies that test what widely used drugs actually do
Heres how it typically looks: Weeks 1 through 4: 2.5 mg weekly (starting dose) Weeks 5 through 8: 5 mg weekly Weeks 9 through 12: 7.5 mg weekly Weeks 13 through 16: 10 mg weekly Weeks 17 and beyond: 12.5 mg or 15 mg weekly (if needed) Each dose increase happens in 2.5 mg increments, and you stay at each level for at least four weeks before moving up
Identification of Estrous Cycle Stage The vagina of the mice was exposed and the external genitalia were wiped with a cotton swab dipped in physiological saline
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