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Discussion The interaction between the gut microbiome and the brain has increasingly become a focus of neuroscience research
Separated proteins were transferred onto Immobilon-P Transfer membranes (MilliporeSigma #IPVH00010), blocked for an hour using 5% milk in TBST, and stained overnight with targeting primary antibodies in 5% milk TBST
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Larger vials suit experienced researchers with established protocols who know exactly how much they'll use over extended periods
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