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Cells were cultured in complete RPMI 1640/GlutaMAX medium (GIBCO, 61870-010) supplemented with 10% heat-inactivated human AB serum (Institut Jacques Boy, 201021334), 1% penicillinstreptomycin (GIBCO, 15140-122), 1% HEPES (GIBCO, 15630-056), 1% sodium pyruvate (GIBCO, 11360-039) and 1% polymyxin B (final concentration 10 g ml 1

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None are currently FDA-approved for T1D, but off-label prescribing has been rising sharply and the clinical data is starting to catch up

DSIP concentrations in the blood exhibit a marked diurnal variation, correlating with circadian rhythms in humans