Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)
However, the ratio of GSSG/GSH did not change significantly in these plants, indicating that there is an alternative pathway(s) to convert GSSG back to GSH in infested resistant plants to maintain a stable GSSG/GSH ratio
Collectively, our findings establish a novel link between the oncogene VRK2 and GSH synthesis metabolism, providing a molecular basis for developing stratified metabolic therapies for PC patients
N-Acetylcysteine (NAC) Supplementation (Most Effective Precursor) Why it works: NAC is a precursor to cysteine, the rate-limiting amino acid in glutathione synthesis