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For example, biased GLP-1R signalling is observed following stimulation with exendin-phe1, which carries a phenylalanine substitution at position 1 of exendin-4, causing a similar reduction in receptor internalisation and increased plasma membrane recycling to GLP-1R V229A (60)
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A protocol built around one splice variant alone may miss half of the biological picture, since activation and sustained signaling are functionally distinct steps in the same repair pathway
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