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2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C

Due to its strong affinity for copper ions, GHK-Cu is commonly utilized in in-vitro and biochemical studies exploring cellular remodeling, peptide-metal interactions, and regulatory mechanisms associated with tissue structure and cellular adaptation

A weak diet that does not include important nutrients moreover antioxidants may lower glutathione

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