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Wilding JPH, Batterham RL, Davies M, Van Gaal LF, Kandler K, Konakli K, Lingvay I, McGowan BM, Oral TK, Rosenstock J, Wadden TA, Wharton S, Yokote K, Kushner RF

Genome-wide identification of genes required for alternative peptidoglycan cross-linking in Escherichia coli revealed unexpected impacts of -lactams

Mouse islets were subsequently washed, purified using a Histopaque gradient (Histopaque-1119 and Histopaque-1083, Sigma-Aldrich) and rested overnight at 37C in a 95% O 2 / 5% CO 2 incubator before performing experiments in mouse islet culture medium (RPMI-1640 with 11 mM D-glucose, supplemented with 10% FBS and 1% penicillin/streptomycin)