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The columns were washed with 15 column volumes of buffer 1 followed by the elution with 200 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C

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11 have shown the efficacy of NACA against L-buthionine-(S,R) sulfoximine (BSO, a glutathione synthesis inhibitor)- induced cataract in wistar rat pups

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Ignoring pH Balance Skin thrives at a pH of 4.55.5

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