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Acknowledgments This work was supported in part by the Japan Study Group of NAFLD (JSG-NAFLD, Kyoto, Japan)

Lipids were separated at a flow rate of 0.3 mL/min and a column temperature of 50C by a gradient elution starting at 10% B increasing in 20 min to 80% B, followed by an increase to 95% B within 4 min, and further 3 min until 100% B is reached and kept for 5 min, followed by a decrease to 10%B within 0.1 min and equilibration at 10% B for 7.9 min 56

HOW IT WORKS Fotona Nd:YAG delivers calibrated laser energy at sub-ablative fluence across the entire treatment zone producing sub-threshold thermal stimulation of melanocytes that progressively reduces their melanin output without triggering the paradoxical melanocyte activation that higher-energy protocols risk in Indian skin

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