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Samples were incubated in 100% methanol overnight and the next day for 3 h (with shaking) in 66% DCM and 33% methanol at room temperature and in 100% DCM 15 min twice (with shaking) to remove traces of methanol
Gearhardt et al., 2011
Although rare, these risks must be communicated clearly to families
any insulin sensitivity improvement is largely secondary to weight loss
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